How to transplanting tissue culture seedling

Preparation

  1. Receive plantlets from the lab, check the code and number plantlets according to the delivery date.
  2. For in-agar shipment, keep the plantlets for 4 – 7 days for hardening at the nursery before planting (plantlets are still in the cup/jar)
  3. Prepare planting medium according to the plantlets varieties and container (pot tray)
  4. Prepare the materials needed for planting, including:
  5. Prepare water in a container and filters to washing media agar attached to the plantlets,
  6. Prepare the container and filters for systemic fungicide solution,
  7. Prepare a container lined with tissue paper to place the plantlets before planting,
  8. Prepare a systemic fungicide solution
  9. Prepare plant growth vitamin solution
  10. Prepare a spatula to remove the plantlets from the cup/jar,
  11. Prepare a hand sprayer filled with water,
  12. Prepare tweezers for planting,
  13. Prepare root growth hormone for unrooted plantlets,
  14. Prepare label.

 

 

Transplanting Process

  1. Before the plantlets are removed, first, fill the cup/jar with water to easily separate the media from the cup/jar. Use a spatula to separate the plantlets and media agar from the cup/jar.
  2. Put the plantlets in a container that has been filled with water. Separate the plantlets one by one from the medium and wash them thoroughly. Do it carefully so that the plantlets are not damaged by washing.
  3. After washing, put the plantlets into a container containing a fungicide solution about 1 minute, then drain it.
  4. Soak in vitamin solution 5 to 10 minutes

Place the drained plantlets in a container lined with tissue paper before planting and grouped according to the size and condition of the plantlets. Cover them again with moistened tissue paper.

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